Search results for "Quantitative Real Time PCR"

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Quantification and viability assays of Toxoplasma gondii in commercial "Serrano" ham samples using magnetic capture real-time qPCR and bioassay techn…

2014

"Serrano" ham is a typical pork product from the Mediterranean area, highly valued for its flavour. To make Serrano ham, pork undergoes a salting and a subsequent fermentation process known as curing. Certain pigs used for meat production are an important source of Toxoplasma gondii infection in humans. We have developed a method for quantifying and assaying the viability of the T. gondii present in commercial Serrano ham samples. A magnetic capture method for the isolation of T. gondii DNA and a qRT-PCR were used to estimate the T. gondii burden in 475 commercial samples of "Serrano" ham in two presentation formats: ham pieces and sliced ham. The infectivity capacity of T. gondii in positi…

SwineFood ContaminationReal-Time Polymerase Chain ReactionMicrobiologyMagneticsMiceparasitic diseasesBioassayAnimalsHumansFood scienceInfectivityMice Inbred BALB CbiologySaltingToxoplasma gondiibiology.organism_classificationVirologyMeat ProductsQuantitative Real Time PCRToxoplasmosis AnimalSpainMediterranean areaBiological AssayToxoplasmaFood ScienceFood microbiology

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PEPhysical Sciences and Engineering
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LSLife Sciences
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